Original Article https://doi.org/10.70084/mru/pmrcc/041.P21

Detection of Microsatellite Markers (LOH) in Urine Samples from Sudanese Patients with Cervical Cancer

Saleh Hussein Ben Sumaidea ^1,2, Ahmed Amin Mohammed Ahmed³, Omar Darkhabani⁴

Affiliations

¹College of Medicine and Health Sciences, Al-Arab University, Yemen. ²College of Medicine and Health Sciences, Hadhramout University, Yemen. ³Department of Obstetrics and Gynecology, Faculty of Medicine, University of Kordofan, El-Obeid, Sudan. ⁴Nahdi Medical Company, Jeddah, Saudi Arabia.

Correspondence to: Saleh Ben Sumaidea. Email: salehhussein721981@gmail.com

Cite: Ben Sumaidea, et al. Detection of Microsatellite Markers (LOH) in Urine Samples from Sudanese Patients with Cervical Cancer.Medical Research Updates Journal 2026;4(2):10-20. https://doi.org/10.70084/mru/pmrcc/041.P21

ABSTRACT

Background: Cervical cancer is one of the leading causes of cancer-related death in women globally. Early discovery of cervical cancer is largely curable; delayed detection lowers survival rates. The current study examined whether microsatellite markers could detect cervical cancer-associated genetic changes in urine samples. Methodology: A total of 23 matched blood and urine samples were taken from Sudanese women with cervical cancer. Four microsatellite markers on separate chromosomal regions—D3S1300 (3p14.2), D3S1260 (3p22.2), D11S528 (11q23.3), and D11S35 (11q22.1)—were evaluated for loss of heterozygosity (LOH), which is known to be common in cervical cancer. To determine the existence of predicted allelic bands, a polymerase chain reaction (PCR) was used, followed by 8% polyacrylamide gel electrophoresis separation. Results: There was no LOH identified in any of the blood samples. In contrast, LOH was detected in urine samples from 18 of 23 individuals (78.3%) at one or more of the four loci studied. The LOH frequencies for these markers were as follows: D3S1300 (3p14.2) in 12 samples (52.2%), D11S35 (11q22.1) in 7 samples (30.4%), D3S1260 (3p22.2) in 8 samples (34.8%), and D11S528 (11q23.3) in 9 samples (39.1%). Two samples showed LOH at two loci, three samples at three loci, and three samples at all four loci, with a predictive value of around 90%. Conclusion: This study shows that LOH at one or more of the four microsatellite markers is related to all kinds of cervical cancer and can be diagnosed from urine samples with 78.3% sensitivity. LOH detection at these loci is more accurate, culturally acceptable, and less invasive for cervical cancer risk assessment.

Keywords: Cervical cancer, microsatellite markers, loss of heterozygosity, Sudan